Turkish Archives of Otorhinolaryngology
Original Article

TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis

1.

Department of Otorhinolaryngology, Mersin University School of Medicine, Mersin, Turkey

2.

Department of Biochemistry, Mersin University School of Medicine, Mersin, Turkey

3.

Department of Biostatistics, Mersin University School of Medicine, Mersin, Turkey

Turk Arch Otorhinolaryngol 2017; 55: 51-56
DOI: 10.5152/tao.2017.2389
Read: 282 Downloads: 126 Published: 03 September 2019

Abstract

Objective: Nasal Polyp (NP) is a benign mass of the paranasal sinuses that protrudes into the nasal cavity. The exact underlying pathogenesis is not known. In this study we aimed to determine the genetic susceptibility of NP formation in relation to TNF-α-308 and IL-1β-511 promoter region gene polymorphisms.

 

Methods: A total of 71 patients with NP with asthma (n=21) or without asthma (n=50) were taken as the study group, and 91 healthy volunteers were taken as the control group. Blood was gathered into EDTA-containing tubes, and patient DNA was extracted. The polymorphisms of the IL-β and TNF-α cytokine genes were analyzed using real time polymerase chain reaction.

 

Results: The GG genotype in the TNF-α-308 region and the CC genotype in the IL-1β-511 region were found to be risk factors for NP formation (OR: 9.2, p=0.007 and OR: 33.3, p=0.001, respectively). Regarding allelic frequencies, the G allele at the TNF-α-308 promoter region was a risk factor for NP formation (OR: 6.06, p<0.001).

 

Conclusion: TNF-α GG genotype in the -308 promoter region and the IL-1β CC genotype in the -511 region are genetic risk factors for NP formation.

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ISSN2667-7466 EISSN 2667-7474