ISSN 2149-3987 | E-ISSN 2149-553X
Original Article
Preliminary Results of a New Experimental Model for Intratympanic Treatment
1 Department of Otorhinolaryngology, Pamukkale University School of Medicine, Denizli, Turkey  
2 Department of Chemistry, Pamukkale University School of Medicine, Denizli, Turkey  
Turk Arch Otorhinolaryngol 2018; 56: 188-192
DOI: 10.5152/tao.2018.3467
Key Words: Inner ear, cochlea, intratympanic injection, dexamethasone
Abstract

 

Objective: Corticosteroids have been applied via transtympanic route for a long time to treat the inner ear disorders. A few animal models were used to answer the questions, “How much drug goes into the inner ear?” and “How far does the drug reach through the scala tympani and/or scala vestibuli?” However, the cerebrospinal fluid contamination poses a major problem. The aims of this study were to create a new sampling model showing the dexamethasone distribution in the inner ear and to provide more reliable data about drug concentrations.

 

Methods: Ten Hartley strain albino guinea pigs that weighted between 400 and 600 g were used. After dexamethasone application to the left ear, they were sacrificed at two time points: after 0.5 hours (Exp 1) and after 2 hours (Exp 2). The temporal bones were immediately dissected and put into liquid nitrogen for freezing. The apex, second turn, and basal turns of the cochlea and vestibule were separated, while the bone was in the frozen state. The samples were prepared and measured with ultraviolet (UV) spectroscopy.

 

Results: The total amount of dexamethasone was statistically higher in the left ear than the control ear. Although the basal turn and vestibule were the most prominent parts, there was no statistical difference between the different parts of the inner ear at 0.5 hours. The vestibule and the apex showed the highest level of dexamethasone at 2 hours.

 

Conclusion: Although the model has some limitations, it can measure dexamethasone concentrations and show the time variability in the inner ear.

 

Cite this article as: Aykal K, Ardıç FN, Tümkaya F, Yücel E, Akarsu M, Kara CO, et al. Preliminary Results of a New Experimental Model for Intratympanic Treatment. Turk Arch Otorhinolaryngol 2018; 56(4): 188-92.

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